Journal: Frontiers in Plant Science
Article Title: The Arabidopsis thaliana E3 Ubiquitin Ligase BRIZ Functions in Abscisic Acid Response
doi: 10.3389/fpls.2021.641849
Figure Lengend Snippet: briz1 and briz2 mutants are alive despite arrested growth. Seeds from BRIZ/briz plants were plated on GM for 15 days (A–I) or 30 days (J–R) before embryos were treated with viability stains. Embryos from Col seeds soaked for 1 h in water were used as controls (S–V) . briz embryos were either heat-treated for 5 min at 98°C or untreated, followed by Sytox staining ( A–C , J–L , S or D–F , M–O , T , respectively) or fluorescein diacetate (FDA) only staining (G–I , P–R , U , V) before confocal microscopy. Heat-treated (S) or untreated Col after Sytox (T) or FDA staining (U) . Heat-treated Col after FDA staining (V) . White bar (V) represents 70 μm. All were visualized at the same scale.
Article Snippet: Fluorescein diacetate (FDA) powder (Invitrogen, F1303) was dissolved in acetone to make a 5 mg/ml stock, then diluted in water to a working concentration of 5 μg/ml.
Techniques: Staining, Confocal Microscopy